Collaboration
Our laboratory is open to scientific and commercial collaboration. Below are some possible areas of cooperation. For inquiries regarding collaboration, please contact the Head of LСIN.
• Development, optimization, and validation of custom enzyme immunoassay (ELISA) and immunochromatographic test systems.
• Investigation of cytotoxicity, immunotoxicity, immunomodulatory, and anti-tumor activity of nanomaterials, proteins, and small organic molecules. Our laboratory maintains cultures of microalgae *Chlorella vulgaris* as well as cell lines such as Jurkat, MCF-7, and THP-1. In addition, we have the capability to work with immune cells isolated from the blood of volunteer donors. We offer a wide range of research methods: from basic viability assays (MTT, quantitative determination of live cell percentage using microscopy or flow cytometry) to advanced physiological studies (surface markers, cytokine production, etc.) and cell differentiation.
• Custom protein expression and purification. We have experience in the production, isolation, and purification of polyclonal and monoclonal antibodies from hybridoma cultures and blood of immunized animals. Among other proteins, we have purified fetoplacental proteins such as alpha-fetoprotein (AFP) and trophoblastic beta-1-glycoprotein (TBG). The laboratory is equipped with a wide range of instruments and sorbents for preparative chromatography (gel filtration, affinity chromatography, ion-exchange chromatography), gel electrophoresis, and Western blotting.
• Application of micro- and nanomaterials with unique properties in immunoassays. We have experience in developing test systems using luminescent nanoparticles, magnetic nanoparticles, and nanozymes (nanoparticles with enzyme-like catalytic activity) in various formats—from instrumental plate-based immunoassays to rapid self-testing kits. If you have interesting nanomaterials that you would like to apply in biomedicine, please contact the Head of the laboratory.
• Analysis of nanoparticle size and zeta potential by dynamic light scattering. We have two particle size analyzers available: ZetaSizer Nano ZS (Malvern) and BeNano Zeta Pro 180 (Bettersize).
